If you are interested in adding flow to your project, don’t hesitate to contact a lab member, we love to collaborate! Alternately, make an appointment with the director, Becca Marti n, and she will be happy to help you with your flow cytometry needs.
![flowjo concatenate flowjo concatenate](http://5b0988e595225.cdn.sohucs.com/images/20181203/4bbf54d090aa4af094d6ae7831cfbe74.jpeg)
Currently, this resource supports over 90 principle investigators at VCU.įlow cytometry is also integral to the study of immunology, and thus each project in the Martin lab utilizes the flow cytometry shared resource in some way. Concatenate with uncompensated parameters when files sh. The Flow Cytometry Shared Resource features a comprehensive suite of instrumentation that facilitates research across the entire university, supporting a diverse array of investigators. Learn best practices for using concatenated files with both compensated and uncompensated parameters. She is assisted by XinYan Pei, MD, PhD, who is a flow cytometry specialist. For example, you may need to create a new FCS file by merging multiple files together (concatenate) to facilitate rare event analysis, or export CSV files that include your workspace structure (gating tree and columns) for use in a downstream application. Figure 5: Sorting data before concatenation. Exporting data from FlowJo is helpful for a variety of tasks. This resource is managed by Julie Farnsworth, who has over 20 years of flow cytometry experience. In Figure 5, the data was sorted by concentration either low to high, or high to low, and the data concatenated.
![flowjo concatenate flowjo concatenate](https://images.squarespace-cdn.com/content/v1/5bc760564d546e3f72b825c1/1556746619240-HWOZUGMZ8M5WBI6V9F4V/ke17ZwdGBToddI8pDm48kM2w9zhiS0mb6Q7bg5kuO0QUqsxRUqqbr1mOJYKfIPR7LoDQ9mXPOjoJoqy81S2I8N_N4V1vUb5AoIIIbLZhVYy7Mythp_T-mtop-vrsUOmeInPi9iDjx9w8K4ZfjXt2diJK1NVXDe3ylzuw7ta_g72dTzb4VO43gf3mKER2L8QlCjLISwBs8eEdxAxTptZAUg/concatenated+events-1.png)
The lab’s principal investigator, Becca Martin, PhD, is director of this shared resource at VCU.